First record of the complete chloroplast genome of Polygonatum infundiflorum (Asparagaceae), a Korean endemic species

Abstract Polygonatum infundiflorum Y.S. Kim, B.U. Oh & C.G. Jang et al. 1998 is a Korean endemic species. This is first report on the complete chloroplast genome sequence of P. infundiflorum. The complete chloroplast genome length was 154,578 bp with a GC content of 37.7%. The large single-copy (LSC) region was 83,527 bp long, and the small single-copy (SSC) region was 18,457 bp long. The paired inverted repeats (IRs) were 26,297 bp and separated the LCS and SSC regions. There were 113 genes, comprising 78 protein-coding genes, four rRNA genes, 30 tRNA genes, and one pseudogene (infA). In total, 16 genes contained one intron, and two genes contained two introns. Phylogenetic analysis suggested that Polygonatum was divided into three sections, each forming a monophyletic group. P. infundiflorum was sister to P. macropodum and formed a monophyletic group with P. inflatum. This study provides basic information for future research and contributes to taxonomic and genetic studies on Polygonatum.


Introduction
Polygonatum Mill. is belongs to Asparagaceae and is divided into three sections (Polygonatum, Verticillata, and Sibirica) based on phylogenetic studies (Meng et al. 2014;Floden and Schilling 2018). These species have been used in traditional medicine and are being actively studied in genetic systematics and marker development (Wujisguleng et al. 2012;Floden and Schilling 2018;Pan et al. 2020;Wang et al. 2022;Yang et al. 2022). Polygonatum infundiflorum Y.S. Kim, B.U. Oh & C.G. Jang et al. 1998 is a Korean endemic species discovered in Pungdo, Ansan-si, Gyeonggi-do (Jang et al. 1998;Oh et al. 2016). It belongs to the sect. Polygonatum, and can be distinguished from related species based on characteristics including curved stem tip, absence of trichomes along the midrib and margin on the abaxial leaf surface, erect stamens, and multicellular trichomes on filaments (Jang et al. 1998;Jang 2002; Figure 1). Although several studies have reported the genetics and complete chloroplast genomes of other Polygonatum species, few studies related to this species have been published. Therefore, we report the complete chloroplast genome of P. infundiflorum in this study to contribute to plant taxonomic identification, genetic diversity, and medicinal uses.
DNA was extracted using a DNeasy Plant Mini Kit (Qiagen Inc., Valencia, CA, USA) and DNA quality and concentration were confirmed through NanoDrop 2000 microspectrophotometer (Thermo Fisher Inc., Waltham, MA, USA). Nextgeneration sequencing (NGS) was generated for sequencing and data analysis using the TruSeq DNA PCR Free (550) library kit on the Illumina MiSeq platform (Macrogen, Inc., Seoul, Korea). The chloroplast sequence was assembled using GetOrganelle with 'embplant_pt' as a reference (Jin et al. 2020) and finally confirmed in Geneious Prime (Kearse et al. 2012). The coding sequence (CDS) and transfer RNA (tRNA) were annotated using GeSeq (Tillich et al. 2017) and tRNAscan-SE software (Lowe and Chan 2016). Genome maps, cis-splicing genes, and trans-splicing genes were generated using CPGview (Liu et al. 2023). The assembled sequence was registered in NCBI GenBank under the accession number OP764686.
We analyzed 30 Polygonatum species together with Maianthemum, Convallaria, and Dracaena of outgroups. We used the MAFFT program in PhyloSuite for 78 CDS sequences that were extracted, concatenated, and aligned (Zhang et al. 2020). We used the ModelFinder program in PhyloSuite (Kalyaanamoorthy et al. 2017) to find the best-fit models for  the maximum likelihood (ML). The best-fit model of ML was TVM þ FþR2 (AICc). The aligned sequence dataset was subjected to ML analysis using IQ-Tree with 10000 bootstrap replicates (Ronquist et al. 2012;Nguyen et al. 2015).
The ML tree for the 78 CDS sequences were analyzed including 30 Polygonatum species and four outgroup species (Maianthemum, Convallaria, and Dracaena). The concatenated CDS length was 64476 bp. In the phylogenetic tree, outgroups branched separately, and Polygonatum was divided into three sections, each forming a monophyletic group. P. infundiflorum was sister to P. macropodum and formed a monophyletic group with P. inflatum. This clade indicated good support (BS ¼ 100% and 88%, respectively), suggesting a close phylogenetic relationship between these three species ( Figure 3).
This study provides the cp genome information of P. infundiflorum, which would contribute to the taxonomic and genetic studies on Polygonatum.

Ethical approval
This study complied with relevant institutional, national, and international guidelines and legislation. This species is not an endangered or protected species, and the collection area is not protected.

Author contributions
Se Ryeong Lee analyzed the data and drafted the manuscript. Sang-Chul Kim collected and analyzed the data. Young-Ho Ha collected the data and conceived the original structure of the review along with Dong Chan Son. All the authors have read and agreed to the submitted version of the manuscript.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Funding
This research was funded by grants from Scientific Research (KNA1-1-13, 14-1) of the Korea National Arboretum, Republic of Korea.

Data availability statement
The genome data that support the findings of this study are available at NCBI (https://www.ncbi.nlm.nih.gov/) under the accession number OP764686. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA897089, SRR22184768, and SAMN31577749, respectively.